Potential of starch as a cheap gelling agent in plant tissue culture media
Cheema, Harvinder K; Patial, Anil
Cheema, Harvinder K. ; Panjab Univ, Dept Bot, Chandigarh 160014, India
55 (1-4) : 215-218 2005
In spite of its higher cost, agar is still used as most frequent gelling agent in commercialisation of plant tissue culture. The potential of tissue culture medium gelled with starch of two different varieties to support seed germination of Glycine max L. and morphogenetic response of various explants (shoot tip, leaf and root) was studied. Explants were obtained from one week old Glycine max L. plants raised in vitro. By analysing various responses, it was found that although there was no significant difference between the two gelling agents (agar and starch) in response to seed germination, yet the starch gelled medium was not found appropriate to replace agar for morphogenetic responses of various explants in culture.
March 14, 2007 in Academics | Permalink
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Comments
I would think somehow the starch might not hold consistency as well as agar would. Just my thoughts.
Posted by: Sabrina | Mar 7, 2009 7:41:57 AM
It seems strange that plants who use starch as a form of storage are unable to utilize this as a source of growth in their other morphological areas. What proteins/nutrients are put in agar anyways?
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Starch can be tempermental to use as a gelling agent. This is why surejel was developed to replace starch to thicken foods such as pie filling. A little more detail on why the starch was rejected would be nice.
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